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Biotechnological platform

Identification of Facility
TitleBiotechnological platform
Key wordsBiotechnology, fermenters, microorganism cultivation, protein, enzyme, purification, characterization
Latest version2013/03/21
DescriptionCultivation of microorganisms and production, purification and characterization of protein and peptides

- liquid fermentation (batch, fed-batch or continuous) of yeasts (various) and bacteria (E.coli)

- monitoring and optimization of culture conditions

- extraction and purification of proteins coming from microorganisms or plant materials

- identification and functional characterization of proteins


Main equipments:

- 8 instrumented fermenters

- downstream processing (centrifugation, tangential-flow micro- and ultrafiltration, expanded-bed chromatography…)

- chromatography, electophoresis (1D, 2D, capillary) separation systems

- specific characterization tests such as chemical, enzymatic, immune tests coupled to picture analysis

Images
Weblinkumr-iate.cirad.fr/equipements/halle-de-biotechnologie
Possibilities and limits1-20 L scale fermentations, multigram protein samples preparation

Application
Application fields Exploration of protein diversity in plant raw material (grappes, cocoa, melon, natural rubber latex, etc.) and through processing .

Production and characterization of enzymes.

Study of interactions between enzymes and polyphenols : biocatalytic systems for polyphenol functionalization…

Application examples 2012 projects :

Selection, study, improvement and use of enzymes – acyltransferases, lipases, galactolipases, cytochromes P450.

Monitoring, control and optimisation of microbial cultures – innovations for monitoring and control of the physiological state of strains.

Wine proteins and peptides.

Characterization of yeast derivatives.

Research for a bio-marker of cocoa fermentation.

Study of melon superoxide dismutase (SOD)

Extraction and characterization of natural rubber proteins.

Production and characterization of recombinant phytases.

Technology sheets that might be related to this facility

Accessibility & Support Service
Access conditions open to external use
Access conditions further comments Accessible to formal partners (collaboration agreement) such as industrial staff, SME staff, academia staff
Languages English, French
Other languages
Training Specific training is necessary to use this infrastructure, Training is provided by the managers of the infrastructure
Training further comments
Technical help staff is available that can help to use this infrastructure
Technical help further comments

Contact data
Contact person Eric Dubreucq, Maeva Subileau
Institute/company INRA - IATE
Country France
Address bât 32, 2 place Pierre Viala 34060 Montpellier cedex 02

Biotechnological platform

Biotechnology, fermenters, microorganism cultivation, protein, enzyme, purification, characterization 2013/03/21


Cultivation of microorganisms and production, purification and characterization of protein and peptides

- liquid fermentation (batch, fed-batch or continuous) of yeasts (various) and bacteria (E.coli)

- monitoring and optimization of culture conditions

- extraction and purification of proteins coming from microorganisms or plant materials

- identification and functional characterization of proteins


Main equipments:

- 8 instrumented fermenters

- downstream processing (centrifugation, tangential-flow micro- and ultrafiltration, expanded-bed chromatography…)

- chromatography, electophoresis (1D, 2D, capillary) separation systems

- specific characterization tests such as chemical, enzymatic, immune tests coupled to picture analysis umr-iate.cirad.fr/equipements/halle-de-biotechnologie 1-20 L scale fermentations, multigram protein samples preparation


Exploration of protein diversity in plant raw material (grappes, cocoa, melon, natural rubber latex, etc.) and through processing .

Production and characterization of enzymes.

Study of interactions between enzymes and polyphenols : biocatalytic systems for polyphenol functionalization… 2012 projects :

Selection, study, improvement and use of enzymes – acyltransferases, lipases, galactolipases, cytochromes P450.

Monitoring, control and optimisation of microbial cultures – innovations for monitoring and control of the physiological state of strains.

Wine proteins and peptides.

Characterization of yeast derivatives.

Research for a bio-marker of cocoa fermentation.

Study of melon superoxide dismutase (SOD)

Extraction and characterization of natural rubber proteins.

Production and characterization of recombinant phytases. open to external use Accessible to formal partners (collaboration agreement) such as industrial staff, SME staff, academia staff English, French

Specific training is necessary to use this infrastructure, Training is provided by the managers of the infrastructure

staff is available that can help to use this infrastructure

Laboratories WikiSysop




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Created by Hte inra on 26 December 2012, at 14:00