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Identification of microorganisms

Identification of Facility
TitleIdentification of microorganisms
AcronymMALDI Biotyper/Microflex-Agroscope Institute of Food Sciences
Key wordsMicroorganisms, food safety, microbial diversity, MALDI-TOF, typing
ManufacturerBruker
ModelMALDI Biotyper, Microflex
DescriptionMALDI biotyping is a well-accepted method for identification of microorganisms. It uses Matrix-Assisted Laser Desorption/Ionisation Time Of Flight Mass Spectrometry (MALDI-TOF MS) coupled to analysis software which identifies and classifies the organism according to its ribosomal protein spectral profile. The method is widely used in clinical diagnostics and has partly replaced conventional microbiological techniques such as biochemical identification (time to result minutes vs. hours or days in classical phenotypic or genotypic identification). Besides bacteria, MS based identification has been shown to be applicable also to yeasts and moulds. This method is used in our laboratory for routine identification of bacteria, yeast and moulds related to food microbiology.
Images
Weblinkhttp://www.agroscope.ch/forschung/05698/05703/05718/index.html?lang=de
Possibilities and limitsMALDI biotyping-based microbial identification is highly reproducible and mostly independent of the culture medium, incubation temperature and growth state. MALDI biotyping has gained much attention over recent years due to its speed, simple handling, cost-effectiveness and high-throughput capabilities.
CertificationISO 9001:2008 / ISO 17025 (STS 223)

Application
Application fields Microorganisms related to food of plant and animal origin. Microbial isolates from plants and environment as well as feed.
Application examples Foodborne pathogens from food of plant and animal origin, feed.
Technology sheets that might be related to this facility

Accessibility & Support Service
Access conditions Accomplishing the requested research as service by the host organization
Access conditions further comments
Languages English, German
Other languages
Training specific training is necessary to use this infrastructure, training is provided by the managers of the infrastructure
Training further comments Only available as service or within collaborative research projects.
Technical help analytical specific staff is dedicated to this infrastructure, staff is available that can help to use this infrastructure
Technical help further comments Specifications will be established for each partnership

Contact data
Contact person Dr. David Drissner
Institute/company Agroscope Institute of Food Sciences
Country Switzerland
Address Schloss 1 8820 W├Ądenswill

MALDI Biotyper, Microflex Identification of microorganisms MALDI Biotyper/Microflex-Agroscope Institute of Food Sciences Microorganisms, food safety, microbial diversity, MALDI-TOF, typing

Bruker MALDI Biotyper, Microflex MALDI biotyping is a well-accepted method for identification of microorganisms. It uses Matrix-Assisted Laser Desorption/Ionisation Time Of Flight Mass Spectrometry (MALDI-TOF MS) coupled to analysis software which identifies and classifies the organism according to its ribosomal protein spectral profile. The method is widely used in clinical diagnostics and has partly replaced conventional microbiological techniques such as biochemical identification (time to result minutes vs. hours or days in classical phenotypic or genotypic identification). Besides bacteria, MS based identification has been shown to be applicable also to yeasts and moulds. This method is used in our laboratory for routine identification of bacteria, yeast and moulds related to food microbiology. http://www.agroscope.ch/forschung/05698/05703/05718/index.html?lang=de MALDI biotyping-based microbial identification is highly reproducible and mostly independent of the culture medium, incubation temperature and growth state. MALDI biotyping has gained much attention over recent years due to its speed, simple handling, cost-effectiveness and high-throughput capabilities. ISO 9001:2008 / ISO 17025 (STS 223)

Microorganisms related to food of plant and animal origin. Microbial isolates from plants and environment as well as feed. Foodborne pathogens from food of plant and animal origin, feed. Accomplishing the requested research as service by the host organization

English, German

specific training is necessary to use this infrastructure, training is provided by the managers of the infrastructure Only available as service or within collaborative research projects. analytical specific staff is dedicated to this infrastructure, staff is available that can help to use this infrastructure Specifications will be established for each partnership Biosafety level 2 laboratory 46.30.62.20




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Created by Kerstin Lienemann on 5 November 2013, at 15:30