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Tools to monitor the peripheral tissues of the wheat grain during fractionation


Key words Grain tissue proportion characterisation, process monitoring, wheat fraction composition, grain milling, dry fractionation
Latest version 2012/11/27
Completed by INRA - IATE

How does it work?

Primary objective This analytical method provides a precise histological composition of the grain fractions.

This accurate knowledge can lead to improved process control, and to the development of new processes, regarding to the commercial, technological and nutritional value of flours.

Working principle Analysis/monitoring of ferulic acid trimer, para-coumaric acid, phytic acid, alkylresorcinol, wheat germ agglutinin and starch, thanks to biochemical measurements.

These specific markers of the different tissues or walls allow to monitor such structures and consequently:

- to describe the processes’ effect, regarding to the characteristics of the raw material (1, 2)

- to establish a link between the fractionation behaviour of the raw material and its structure & composition (3)

- to understand and control the tissues and constituents sorting during fractionation (4-8)

Additional effects This analytical method could replace the current reference method for flour purity (ashes in France, which does not provide any specific information about technological nor nutritional values of the flour)
Important process parameters
Important product parameters

What can it be used for?

Products Wheat (soft, hard, durum) (7) and other cereals with restrictions (biochemical markers specificity and location has to be determined in other cereals).

Already tested on barley (8).

Operations Milling, debranning, new tissue separation process
Solutions for short comings Commercial, technological and nutritional value of wheat

What can it NOT be used for?

Products any other product than cereals
Operations any other operation than separation
Other limitations not known
Risks or hazards No information currently available


Maturity Currently reference analytical methods, easily adaptable to control laboratories but time consuming.

A much simpler and faster method is being developed to be inserted in industrial sites (UNDER PROGRESS). Indeed, the biochemical measurement that allows the analysis/monitoring of interesting compounds shall be replaced by faster methods such as spectrometry (9) or measurement of specific antibodies (e.g. ELISA technique)

Modularity /Implementation The method could probably not be developed for on line analysis
Consumer aspects This method could be used to develop new methods in order to follow the tissue origin of fibres. Indeed the nature of fibre is different from distinct tissues. Potentially it could help to improve the consumers’ health (particularly on colon issues).
Legal aspects No information currently available, please check local legislation
Environmental aspects No information currently available

Further Information

Institutes INRA - IATE
Companies (not commercially available yet)
References (1) Antoine C., Peyron S., Lullien-Pellerin V., Abecassis J. and Rouau X., 2004. Wheat bran tissue fractionation using biochemical markers. J cereal Sci. 39(3), 387-393

(2) Antoine C., Lullien-Pellerin V., Abecassis J. and Rouau X., 2004. Effect of wheat bran ball milling on fragmentation and marker extractability of the aleurone layer. J cereal Sci. 40(3), 275-282

(3) Greffeuille V., Abecassis J., L’Helgouac’h C. and Lullien-Pellerin V., 2005. Differences in the aleurone layer fate between hard and soft common wheats at grain milling. Cereal Chem. 82 (2), 138-143.

(4) Hemery Y., Rouau X., Lullien-Pellerin V., Barron C. and Abecassis J., 2007. Dry processes to develop wheat fractions and products with enhanced nutritional quality. J cereal Sci. 46(3), 327-347

(5) Hemery Y., Rouau X., Lullien-Pellerin V., Abecassis J., Samson M.F., Per Aman, Walter von Reding, Cäcilia Spoerndli and Barron C., 2009. Biochemical markers: Efficient tools for the assessment of wheat grain tissue proportion in milling fractions. J cereal Sci. 46(3), 55-64

(6) Barron C., Samson M.F., Lullien-Pellerin V., Rouau X. 2011. Wheat grain tissue proportions in milling fractions using biochemical marker measurements: Application to different wheat cultivars. J. Cereal Sci. 53 (3): 306-311.

(7) Rios G., Pinson-Gadais L., Abecassis J., Zakhia-Rozis N., Lullien-Pellerin V., 2009. Assessment of dehulling efficiency to reduce deoxynivalenol and Fusarium level in durum wheat grains. J. Cereal Sci. 49, 387-392.

(8) Mayolles J.E., Lullien-Pellerin V., Corbineau F., Boivin P., Guillard V., 2012. Water diffusion and enzyme activities during malting of barley grains: A relationship assessment. J. Food Eng. 109, 358-365.

(9) Barron C., 2011. Prediction of Relative Tissue Proportions in Wheat Mill Streams by Fourier Transform Mid-infrared Spectroscopy. J. Agric. Food Chem 59 (19), 10442-10447.

Mills 2.1.3 chemical, biological separation other Interviewing the researchers of UMR 1208 IATE (INRA, Montpellier, France): Xavier Rouau, Valérie Lullien-Pellerin WikiSysop :Template:Review document :Template:Review status

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Created by Hte inra on 23 September 2011, at 10:42